Although seed treatments should inhibit most fungal growth, this can be untrue when applied incorrectly. The indicated seed (yellow arrow) shows typical blackleg infection symptoms (silvery loose hyphae and pink ooze from black pycnidia), and was confirmed by PCR to be P. big.
Sterilant:
None
Sterilant time:
N/A
Growth time:
7 days
Lighting:
3:1 (fluorescent:nUV), within 60cm of light. Alternating light/dark in 12 hr intervals