Overview
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Scientific name
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Ustilago tritici |
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Genus
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Ustilago |
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EPPO code
|
USTINT |
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Common name
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Loose smut of wheat |
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Synonyms
|
Ustilago nuda f. sp. tritici |
Description
From ISTA's Common Laboratory Seed Health Testing Methods for Detecting Fungi:
After isolating the embryos from the seed, soaking, clearing and staining is required (staining is not an option). It is difficult to see the blue-stained mycelium in the embryo, so you must rotate/flip the embryos to closely observe the scutellum. In some infected embryos, only a few hyphae are observed, while in others the whole scutellum is covered by sparse to heavy mycelium.
Images of Ustilago tritici
Ustilago tritici on Triticum spp.
Image credits: SASA, FR
Wheat embryo extracted by modified ISTA method 7-013a showing Ustilago tritici hyphae, stained with Methyl blue
Ustilago tritici on Triticum spp.
Image credits: SASA, FR
Wheat embryo extracted by modified ISTA method 7-013a showing Ustilago tritici hyphae, stained with Methyl blue