Epichloe spp. on Lolium spp.
Image credits: SASA, GB
Image of antigen blotter showing positive reaction obtained from Festulolium seeds infected with Epichloe species endophyte. Immunoblot kit used: Agrinostics Ltd, Phytoscreen, Epichloe seed (Cat # ENDO 7971). For this kit, blue staining indicates infected seed. For this particular sample, 74% of the 100 plated seeds were positive. Top right indicates the controls from the kit, dark spot is positive.
growth time: 7 | temperature: 20oC | lighting: 12 hours NUV | plate size: 90mm | agar: Carnation Leaf Agar ( Oxoid Technical No 3 )